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KMID : 0616120100410040313
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Korean Journal of Pharmacognosy
2010 Volume.41 No. 4 p.313 ~ p.318
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Simultaneous Determination of Six Bioactive Components in Guibi-tang by HPLC-DAD
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Yang Hye-Jin
Weon Jin-Bae
Ma Jin-Yeul
Ma Choong-Je
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Abstract
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Guibi-tang, a traditional herbal medicine, is used for anti-oxidant, anti-osteoporosis, hemostasis and gastroprotection. To develop an analysis method of simultaneous determination of six compounds, swertisin, decursinol, glycyrrhizin, 6-gingerol, costunolide and decursin in Guibi-tang, a high performance liquid chromatography was used with diode array detector. Six bioactive components were separated on a SHISEIDO C18 column (5 μm, 4.6 mm I.D.×250 mm) with column temperature 30 o C. The gradient elution was composed of water with 0.1% trifluoroacetic acid (TFA) and acetonitrile. UV wavelength was set at 230 nm, 254 nm and 330 nm, respectively. Calibration curve showed good linear regression (R 2 > 0.9999). The limits of detection (LOD) and the limits of quantification (LOQ) ranged in 0.03 - 0.23 μg/ml and 0.08 - 0.70 μg/ ml, respectively. The RSD values of intra- and inter-day test were in the range of 0.03 ~ 0.96% and 0.01 ~ 1.46%, respectively. The evaluated results of accuracy test were varied from 92.28% ~ 105.14% with RSD < 1.60%. In conclusion, this developed simultaneous determination method was accuracy and sensitive to the quality evaluation of Guibi-tang.
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KEYWORD
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Guibi-tang, Simultaneous determination, High performance liquid chromatography (HPLC), Validation
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